The Eocene-Oligocene boundary in Mississippi and Alabama has been traditionally placed between the Shubuta Member of the Yazoo Formation and the overlying Red Bluff Formation (or its carbonate facies equivalent, the Bumpnose Limestone). Consequently, the presence of Eocene planktonic foraminifers in the Red Bluff and Bumpnose has long been attributed to reworking. To test the validity of this hypothesis, samples were collected on both sides of the boundary from the upper "Shubuta" and Bumpnose units at Little Stave Creek, Alabama, and they were examined for both calcareous nannofossil and planktonic foraminiferal content. The calcareous nannofossil assemblage, preserved in the matrix from inside hand-picked specimens of Hantkenina from both units, was demonstrably older t an the calcareous nannofossil assemblage from the surrounding sediment. Thus, at least some of the Hantkenina specimens in both the "Shubuta" and Bumpnose are indeed reworked, which not only confirms the original hypothesis regarding reworking within the Red Bluff and Bumpnose, but also indicates that the last occurrence of Hantkenina, the "Shubuta"-Bumpnose contact, and the Eocene-Oligocene boundary in the U.S. Gulf Coast may not be equivalent.

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