Most water development schemes in Jordan have paid limited attention to the chemistry of water and its implication on water origin, movement, and evolution. Various aspects of water quality have also been ignored in recent projects. Chemical characteristics of groundwater in a major catchment area adjacent to the Maqarin dam in northern Jordan have been investigated. Because water from this area is an intended major source of water for Jordan, it is vital to understand the nature of the chemistry of the springs in the area, as well as the potential water-rock interaction. The study area is underlain by sedimentary rocks of Upper Cretaceous age. This study evaluates the water quality of 12 springs from aquifers within the bituminous marl formation and chalky limestone formation (aquifers B-3 and B-4, respectively) situated in the surrounding area of the Maqarin dam site on the Yarmouk river. Analyses show that the water of the springs are characterized as alkaline earth-biocarbonate chemical type, consistent with the calcareous lithology of the aquifers. Constituents contained in all water from springs sampled are within or below World Health Organization (WHO) standards set for domestic purposes.