Decimeter-scale sampling of the Cambrian and the lowermost Ordovician (Sauk megasequence) rocks of the Llano uplift, Texas, has produced a finely resolved biostratigraphic framework based primarily on trilobites and conodonts. Systematically collected trilobites of the Llano Uplift allow recognition of 13 biozones that extend from the Bolaspidella Biozone (Cambrian System, Marjuman Stage) through the Symphysurina Zone (Ordovician System, upper Skullrockian Stage). Systematic collection of conodonts has produced specimens assignable to 13 zones that range from the Proconodontus tenuiserratus Zone (Cambrian System, Sunwaptan Stage) through the Rossodus manitouensis Zone (Ordovician System, upper Skullrockian Stage). The base of the Ordovician System in the Llano uplift, as elsewhere, has been identified by the lowest occurrence of the conodont Iapetognathus fluctivagus and is closely approximated by the lowest occurrences of the cosmopolitan trilobite Juyjuyaspis and the Laurentian trilobite Symphysurina “bulbosa.” Although the overlying Ordovician strata of the Ellenburger Group have not been systematically sampled, scattered trilobite collections do establish the approximate positions of the base of the Stairsian Stage (based on Paraplethopeltis) and the base of the Jeffersonian Stage (based on Rananasus and Jeffersonia) in the Tanyard and Honeycut Formations, respectively.