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Abstract

DOI:10.1306/13171269St593042

Indicative Microorganisms as a Tool for Testing the Underground Storage of Carbon Dioxide

Radoslaw Tarkowski,1 Wojciech Krolik,2 Barbara Uliasz-Misiak,3 Wieslaw Barabasz4

1Mineral and Energy, Economy Research Institute of Polish Academy of Sciences, Krakow, Poland
2Mineral and Energy, Economy Research Institute of Polish Academy of Sciences, Krakow, Poland
3Mineral and Energy, Economy Research Institute of Polish Academy of Sciences, Krakow, Poland
4Agriculture University of Krakow, Department of Microbiology, Krakow, Poland

ACKNOWLEDGMENTS

This chapter is partly based on results obtained in the course of the CO2SINK Project of the Sixth European Union Frame Programme (EU 6th FP, contract SES6-CT-2004-502599).

ABSTRACT

The research results presented in this chapter concern the possible usage of microorganisms in measurements of CO2 in subsurface environment. Microbiological tests of Previous HitsoilNext Hit samples from the Muszyna region, which is an area of natural CO2 exhalations (southern Poland), and the Ketzin region, which is an area containing an underground CO2 storage facility (near Berlin, Germany), were run. Quantitative and qualitative compositional analysis of microflora present in investigated samples allowed the selection of bacteria whose proliferation reflects the availability of CO2. Clostridium kluyveri bacteria and the nitrification process can be applied as indicators to estimate the amount of CO2 in Previous HitsoilNext Hit gas. Thiobacillus bacteria also appear to be valuable as indicators of increased amounts of CO2 in Previous HitsoilTop gas. Based on changes in the Thiobacillus population present on pyrite surfaces, we suggest herein that a biosensor can be constructed to measure the quantity of CO2.

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